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Prodromou, Chrisostomos, Savva, Renos and Driscoll, Paul C (2007) DNA fragmentation-based combinatorial approaches to soluble protein expression: Part I. Generating DNA fragment libraries. Drug Discovery Today, 12 (21-22). pp. 931-938. ISSN 1359-6446
Full text not available from this repository.Abstract
In addressing a new drug discovery target, the generation of tractable protein substrates for functional and structural analyses can represent a significant hurdle. Traditional approaches rely on protein expression trials of multiple variants in various systems, frequently with limited success. The increasing knowledge base derived from genomics and structural proteomics initiatives assists the bioinformatics-led design of these experiments. Nevertheless, for many eukaryotic polypeptides, particularly those with relatively few homologues, the generation of useful protein products can still be a major challenge. This review describes the basis of efforts to forge an alternative 'domain-hunting' paradigm, based upon combinatorial sampling of expression construct libraries derived by fragmentation of the encoding DNA template, namely the methods and considerations in generating fragment length DNA from target genes. An accompanying review focuses upon the expression screening of such combinatorial DNA libraries for the sampling of the corresponding set of protein fragments.
| Item Type: | Article |
|---|---|
| Keywords: | Combinatorial Chemistry Techniques/*methods *DNA Fragmentation Drug Design *Gene Library Polymerase Chain Reaction Proteins/*chemistry |
| Schools and Departments: | School of Life Sciences > Biochemistry |
| Subjects: | Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry T Technology > TP Chemical technology > TP0248.13 Biotechnology |
| Depositing User: | Chrisostomos Prodromou |
| Date Deposited: | 24 Feb 2015 17:49 |
| Last Modified: | 25 Feb 2015 09:28 |
| URI: | http://srodev.sussex.ac.uk/id/eprint/44366 |