Savva, Renos, Prodromou, Chrisostomos and Driscoll, Paul C (2007) DNA fragmentation based combinatorial approaches to soluble protein expression Part II: library expression, screening and scale-up. Drug Discovery Today, 12 (21-22). pp. 939-947. ISSN 1359-6446

Full text not available from this repository.

Abstract

In this second of a two-part review encompassing random, combinatorial methods for soluble protein 'domain hunting', we focus upon the expression screening from DNA fragment libraries. Given a library of domain length-encoding DNA fragments assembled in expression vectors, it is necessary to devise reliable means to screen the sample DNA fragment population to find those that express stable, soluble target protein fragments, suitable for the required downstream aims. This review summarizes a variety of alternative strategies that have been employed to identify such stable truncates of full-length proteins. In addition, we review measures that can determine the quality of the expressed protein, the likely reliability of these measures, and the apparent extent of their application within the featured studies.

Item Type:	Article
Keywords:	Combinatorial Chemistry Techniques/*methods *DNA Fragmentation *Gene Library Phosphatidylinositol 3-Kinases/chemistry Protein Folding Protein Structure, Tertiary Proteins/*chemistry Silent Information Regulator Proteins, Saccharomyces cerevisiae/chemistry Solubility Telomerase/chemistry Viral Proteins/chemistry
Schools and Departments:	School of Life Sciences > Biochemistry
Subjects:	Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry T Technology > TP Chemical technology > TP0248.13 Biotechnology
Depositing User:	Chrisostomos Prodromou
Date Deposited:	25 Feb 2015 09:40
Last Modified:	25 Feb 2015 09:40
URI:	http://srodev.sussex.ac.uk/id/eprint/44375

📧 Request an update