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Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

journal contribution
posted on 2023-06-15, 14:02 authored by Mark Osborne, Remi Boulineau
A technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Chemical Communications

ISSN

1359-7345

Publisher

Royal Society of Chemistry

Issue

1

Volume

49

Page range

5559-5561

Department affiliated with

  • Chemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2013-05-13

First Compliant Deposit (FCD) Date

2013-05-10

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