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Osborne, Mark and Boulineau, Remi (2013) Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores. Chemical Communications, 49 (1). pp. 5559-5561. ISSN 1359-7345
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Official URL: http://dx.doi.org/10.1039/C3CC42072H
Abstract
A technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.
Item Type: | Article |
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Schools and Departments: | School of Life Sciences > Chemistry |
Subjects: | Q Science > QD Chemistry > QD0071 Analytical chemistry Q Science > QD Chemistry > QD0450 Physical and theoretical chemistry |
Depositing User: | Mark Osborne |
Date Deposited: | 13 May 2013 08:34 |
Last Modified: | 11 Sep 2017 17:43 |
URI: | http://srodev.sussex.ac.uk/id/eprint/44688 |
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