Estimating the number of double-strand breaks formed during meiosis from partial observation

Toyoizumi, Hiroshi and Tsubouchi, Hideo (2012) Estimating the number of double-strand breaks formed during meiosis from partial observation. Journal of Computational Biology, 19 (12). pp. 1277-1283. ISSN 1066-5277

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Abstract

Analyzing the basic mechanism of DNA double-strand breaks (DSB) formation during meiosis is important for understanding sexual reproduction and genetic diversity. The location and amount of meiotic DSBs can be examined by using a common molecular biological technique called Southern blotting, but only a subset of the total DSBs can be observed; only DSB fragments still carrying the region recognized by a Southern blot probe are detected. With the assumption that DSB formation follows a nonhomogeneous Poisson process, we propose two estimators of the total number of DSBs on a chromosome: (1) an estimator based on the Nelson-Aalen estimator, and (2) an estimator based on a record value process. Further, we compared their asymptotic accuracy.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Deeptima Massey
Date Deposited: 23 May 2013 11:44
Last Modified: 23 May 2013 11:44
URI: http://srodev.sussex.ac.uk/id/eprint/45089
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