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mRNA encoding WAVE-Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration
journal contribution
posted on 2023-06-08, 16:10 authored by Mark Willett, Michele Brocard, Hilary J Pollard, Simon MorleyDuring cell spreading, mammalian cells migrate using lamellipodia formed from a large dense branched actin network which produces the protrusive force required for leading edge advancement. The formation of lamellipodia is a dynamic process and is dependent on a variety of protein cofactors that mediate their local regulation, structural characteristics and dynamics. In the present study, we show that mRNAs encoding some structural and regulatory components of the WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex are localized to the leading edge of the cell and associated with sites of active translation. Furthermore, we demonstrate that steady-state levels of ArpC2 and Rac1 proteins increase at the leading edge during cell spreading, suggesting that localized protein synthesis has a pivotal role in controlling cell spreading and migration.
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Publication status
- Published
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- Published version
Journal
The Biochemical JournalISSN
1470-8728Publisher
Portland PressExternal DOI
Issue
1Volume
452Page range
45-55Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2013-11-02First Compliant Deposit (FCD) Date
2013-11-02Usage metrics
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