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Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic division

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posted on 2023-06-08, 17:42 authored by Alice CopseyAlice Copsey, Shangming Tang, Philip W Jordan, Hannah Blitzblau, Sonya Newcombe, Andrew Chi-ho Chan, Louise Newnham, Zhaobo Li, Steve Gray, Alex Herbert, Prakash Arumugam, Andreas Hochwagen, Neil Hunter, Eva Hoffmann
During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutL? complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4Eme1. Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe.

Funding

BB/G00353X/1; BBSRC

History

Publication status

  • Published

File Version

  • Published version

Journal

PLoS Genetics

ISSN

1553-7390

Publisher

Public Library of Science

Issue

12

Volume

9

Article number

e1004071

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

PWJ was funded by BBSRC grant (BB/G00353X/1). SG was supported by a MRC Centenary Award. EH is an EMBO Young Investigator and MRC Senior Non-clinical Research Fellow.

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2014-07-02

First Open Access (FOA) Date

2014-07-02

First Compliant Deposit (FCD) Date

2014-07-01

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