journal.pgen.1004071.pdf (7.62 MB)
Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic division
journal contribution
posted on 2023-06-08, 17:42 authored by Alice CopseyAlice Copsey, Shangming Tang, Philip W Jordan, Hannah Blitzblau, Sonya Newcombe, Andrew Chi-ho Chan, Louise Newnham, Zhaobo Li, Steve Gray, Alex Herbert, Prakash Arumugam, Andreas Hochwagen, Neil Hunter, Eva HoffmannDuring meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutL? complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4Eme1. Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe.
Funding
BB/G00353X/1; BBSRC
History
Publication status
- Published
File Version
- Published version
Journal
PLoS GeneticsISSN
1553-7390Publisher
Public Library of ScienceExternal DOI
Issue
12Volume
9Article number
e1004071Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
PWJ was funded by BBSRC grant (BB/G00353X/1). SG was supported by a MRC Centenary Award. EH is an EMBO Young Investigator and MRC Senior Non-clinical Research Fellow.Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2014-07-02First Open Access (FOA) Date
2014-07-02First Compliant Deposit (FCD) Date
2014-07-01Usage metrics
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