Garg, Mansi, Gurung, Resham L, Mansoubi, Sahar, Ahmed, Jubed O, Davé, Anoushka, Watts, Felicity Z and Bianchi, Alessandro (2014) Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association. EMBO Reports. ISSN 1469-3178

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Abstract

Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13
SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association,and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.

Item Type:	Article
Additional Information:	Keywords: CST; Stn1; telomeres; Tpz1; SUMO
Schools and Departments:	School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects:	Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Depositing User:	Gee Wheatley
Date Deposited:	11 Jul 2014 09:10
Last Modified:	23 Jul 2020 09:20
URI:	http://srodev.sussex.ac.uk/id/eprint/49241

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