Site-specific human histone H3 methylation stability: fast K4me3 turnover

Zheng, Yupeng, Tipton, Jeremiah D, Thomas, Paul M, Kelleher, Neil L and Sweet, Steve M M (2014) Site-specific human histone H3 methylation stability: fast K4me3 turnover. Proteomics. ISSN 1615-9853

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We employ stable-isotope labeling and quantitative mass spectrometry to track histone methylation
stability. We show that H3 trimethyl K9 and K27 are slow to be established on new
histones and slow to disappear from old histones, with half-lives of multiple cell divisions. By
contrast, the transcription-associated marks K4me3 and K36me3 turn over far more rapidly,
with half-lives of 6.8 h and 57 h, respectively. Inhibition of demethylases increases K9 and
K36 methylation, with K9 showing the largest and most robust increase. We interpret different
turnover rates in light of genome-wide localization data and transcription-dependent
nucleosome rearrangements proximal to the transcription start site.

Item Type: Article
Additional Information: Keywords: Cell biology / Chromatin / Demethylation / Heterochromatin / Methylation turnover / SILAC / Transcription
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Depositing User: Gee Wheatley
Date Deposited: 07 Aug 2014 12:06
Last Modified: 23 Jul 2020 09:25

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