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Use of a pooled clone method to isolate a novel Bacillus thuringiensis Cry2A toxin with activity against Ostrinia furnacalis

journal contribution
posted on 2023-06-08, 18:12 authored by Changlong Shu, Jingtao Zhang, Guihua Chen, Gemei Liang, Kanglai He, Neil CrickmoreNeil Crickmore, Dafang Huang, Jie Zhang, Fuping Song
A pooled clone method was developed to screen for cry2A genes. This metagenomic method avoids the need to analyse isolated Bacillus thuringiensis strains by performing gene specific PCR on plasmid-enriched DNA prepared from a pooled soil sample. Using this approach the novel holotype gene cry2Ah1 was cloned and characterized. The toxin gene was over-expressed in Escherichia coli Rosetta (DE3) and the expressed toxin accumulated in both the soluble and insoluble fractions. The soluble Cry2Ah1 was found to have a weight loss activity against Ostrinia furnacalis, and a growth inhibitory activity to both Cry1Ac-susceptible and resistant Helicoverpa armigera populations.

History

Publication status

  • Published

Journal

Journal of Invertebrate Pathology

ISSN

0022-2011

Publisher

Elsevier

Issue

1

Volume

114

Page range

31-33

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2014-09-05

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