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Generation and characterisation of human saphenous vein endothelial cell lines
journal contribution
posted on 2023-06-08, 19:45 authored by Ariane Scoumanne, Tahereh Kalamati, Jill Moss, Janet T Powell, Martin Gosling, Nessa CareyPrimary human endothelial cells have a finite life span in vitro. After 3-4 passages, they tend to de-differentiate and eventually reach senescence. This limits their use in studies of endothelial cell function. To overcome this, we have developed human saphenous vein endothelial cell lines (HSVEC lines). Two cell lines were produced by infection with pZipSVtsA58-U19 which encodes the simian virus 40 large T-antigen, and one cell line was obtained by transfection with pLXSN16E6E7, which encodes the human papillomavirus type 16 E6 and E7 genes. Two of the three HSVEC lines exhibited an extended life span in vitro and retained characteristic endothelial 'cobblestone' morphology. These cell lines expressed the known endothelial markers CD31 and vascular endothelial cadherin, and were able to bind Ulex europaeus lectin I, but they did not retain the expression of von Willebrand factor. Furthermore, one cell line was able to functionally up-regulate the expression of intercellular adhesion molecule-1 in response to stimulation with tumor necrosis factor a and was also able to incorporate acetylated low-density lipoprotein. Our results suggest that this latter HSVEC line will provide a useful resource to investigate selected responses of the vascular endothelium to physiological and pathological situations. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
History
Publication status
- Published
Journal
AtherosclerosisISSN
0021-9150Publisher
ElsevierExternal DOI
Issue
1Volume
160Page range
59-67Department affiliated with
- Chemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-01-27Usage metrics
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