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Order and disorder in crystals of hexameric NTPases from dsRNA bacteriophages

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posted on 2023-06-08, 19:50 authored by Erika ManciniErika Mancini, J M Grimes, R Malby, G C Sutton, D E Kainov, J T Juuti, E V Makeyev, R Tuma, D H Bamford, D I Stuart
The packaging of genomic RNA in members of the Cystoviridae is performed by P4, a hexameric protein with NTPase activity. Across family members such as F6, F8 and F13, the P4 proteins show low levels of sequence identity, but presumably have similar atomic structures. Initial structure-determination efforts for P4 from F6 and F8 were hampered by difficulties in obtaining crystals that gave ordered diffraction. Diffraction from crystals of full-length P4 showed a variety of disorder and anisotropy. Subsequently, crystals of F13 P4 were obtained which yielded well ordered diffraction to 1.7 Å. Comparison of the packing arrangements of P4 hexamers in different crystal forms and analysis of the disorder provides insights into the flexibility of this family of proteins, which might be an integral part of their biological function.

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Publication status

  • Published

File Version

  • Published version

Journal

Acta Crystallographica Section D: Biological Crystallography

ISSN

0907-4449

Publisher

International Union of Crystallography

Issue

12

Volume

59

Page range

2337-2341

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-29

First Open Access (FOA) Date

2015-01-29

First Compliant Deposit (FCD) Date

2015-01-29

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