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Order and disorder in crystals of hexameric NTPases from dsRNA bacteriophages
journal contribution
posted on 2023-06-08, 19:50 authored by Erika ManciniErika Mancini, J M Grimes, R Malby, G C Sutton, D E Kainov, J T Juuti, E V Makeyev, R Tuma, D H Bamford, D I StuartThe packaging of genomic RNA in members of the Cystoviridae is performed by P4, a hexameric protein with NTPase activity. Across family members such as F6, F8 and F13, the P4 proteins show low levels of sequence identity, but presumably have similar atomic structures. Initial structure-determination efforts for P4 from F6 and F8 were hampered by difficulties in obtaining crystals that gave ordered diffraction. Diffraction from crystals of full-length P4 showed a variety of disorder and anisotropy. Subsequently, crystals of F13 P4 were obtained which yielded well ordered diffraction to 1.7 Å. Comparison of the packing arrangements of P4 hexamers in different crystal forms and analysis of the disorder provides insights into the flexibility of this family of proteins, which might be an integral part of their biological function.
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Publication status
- Published
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- Published version
Journal
Acta Crystallographica Section D: Biological CrystallographyISSN
0907-4449Publisher
International Union of CrystallographyExternal DOI
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12Volume
59Page range
2337-2341Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-01-29First Open Access (FOA) Date
2015-01-29First Compliant Deposit (FCD) Date
2015-01-29Usage metrics
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