Mancini, E J and Fuller, S D (2000) Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus. Acta Crystallographica Section D: Biological Crystallography, 56 (10). pp. 1278-1287. ISSN 0907-4449

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Abstract

The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Biochemistry
Subjects:	Q Science
Depositing User:	Tom Gittoes
Date Deposited:	29 Jan 2015 10:19
Last Modified:	26 Mar 2017 16:38
URI:	http://srodev.sussex.ac.uk/id/eprint/52568

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