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Diversity in Acinetobacter baumannii isolates from paediatric cancer patients in Egypt

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posted on 2023-06-08, 20:10 authored by Leena Al-HassanLeena Al-Hassan, H El Mehallawy, S G B Amyes
Acinetobacter baumannii is an important nosocomial pathogen, commonly causing infections in immunocompromised patients. It is increasingly reported as a multidrug-resistant organism, which is alarming because of its capability to resist all available classes of antibiotics including carbapenems. The aim of this study was to examine the genetic and epidemiological diversity of A. baumannii isolates from paediatric cancer patients in Egypt, by sequencing the intrinsic blaOXA -51-like gene, genotyping by pulsed-field gel electrophoresis and multi-locus sequence typing in addition to identifying the carbapenem-resistance mechanism. Results showed a large diversity within the isolates, with eight different blaOXA -51-like genes, seven novel sequence types and only 28% similarity by pulsed-field gel electrophoresis. All three acquired class-D carbapenemases (OXA-23, OXA-40 and OXA-58) were also identified among these strains correlating with resistance to carbapenems. In addition, we report the first identification of ISAba2 upstream of blaOXA -51-like contributing to high-level carbapenem resistance. This indicates the presence of several clones of A. baumannii in the hospitals and illustrates the large genetic and epidemiological diversity found in Egyptian strains.

History

Publication status

  • Published

File Version

  • Published version

Journal

Clinical Microbiology and Infection

ISSN

1198-743X

Publisher

Wiley-Blackwell

Issue

11

Volume

19

Page range

1082-1088

Department affiliated with

  • Global Health and Infection Publications

Research groups affiliated with

  • Brighton and Sussex Centre for Global Health Research Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-03-02

First Open Access (FOA) Date

2015-03-02

First Compliant Deposit (FCD) Date

2015-03-02

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