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Diversity in Acinetobacter baumannii isolates from paediatric cancer patients in Egypt
journal contribution
posted on 2023-06-08, 20:10 authored by Leena Al-HassanLeena Al-Hassan, H El Mehallawy, S G B AmyesAcinetobacter baumannii is an important nosocomial pathogen, commonly causing infections in immunocompromised patients. It is increasingly reported as a multidrug-resistant organism, which is alarming because of its capability to resist all available classes of antibiotics including carbapenems. The aim of this study was to examine the genetic and epidemiological diversity of A. baumannii isolates from paediatric cancer patients in Egypt, by sequencing the intrinsic blaOXA -51-like gene, genotyping by pulsed-field gel electrophoresis and multi-locus sequence typing in addition to identifying the carbapenem-resistance mechanism. Results showed a large diversity within the isolates, with eight different blaOXA -51-like genes, seven novel sequence types and only 28% similarity by pulsed-field gel electrophoresis. All three acquired class-D carbapenemases (OXA-23, OXA-40 and OXA-58) were also identified among these strains correlating with resistance to carbapenems. In addition, we report the first identification of ISAba2 upstream of blaOXA -51-like contributing to high-level carbapenem resistance. This indicates the presence of several clones of A. baumannii in the hospitals and illustrates the large genetic and epidemiological diversity found in Egyptian strains.
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Publication status
- Published
File Version
- Published version
Journal
Clinical Microbiology and InfectionISSN
1198-743XPublisher
Wiley-BlackwellExternal DOI
Issue
11Volume
19Page range
1082-1088Department affiliated with
- Global Health and Infection Publications
Research groups affiliated with
- Brighton and Sussex Centre for Global Health Research Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-03-02First Open Access (FOA) Date
2015-03-02First Compliant Deposit (FCD) Date
2015-03-02Usage metrics
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