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Keratin dynamics: modeling the interplay between turnover and transport

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journal contribution
posted on 2023-06-08, 20:33 authored by Stéphanie Portet, Anotida MadzvamuseAnotida Madzvamuse, Andy Chung, Rudolf E Leube, Reinhard Windoffer
Keratin are among the most abundant proteins in epithelial cells. Functions of the keratin network in cells are shaped by their dynamical organization. Using a collection of experimentally-driven mathematical models, different hypotheses for the turnover and transport of the keratin material in epithelial cells are tested. The interplay between turnover and transport and their effects on the keratin organization in cells are hence investigated by combining mathematical modeling and experimental data. Amongst the collection of mathematical models considered, a best model strongly supported by experimental data is identified. Fundamental to this approach is the fact that optimal parameter values associated with the best fit for each model are established. The best candidate among the best fits is characterized by the disassembly of the assembled keratin material in the perinuclear region and an active transport of the assembled keratin. Our study shows that an active transport of the assembled keratin is required to explain the experimentally observed keratin organization.

Funding

InCeM: Research Training Network on Integrated Component Cycling in Epithelial Cell Motility; G1546; EU-Horizon2020 MSCA-ITAN

Mathematical Modelling and Analysis of Spatial Patterning on Evolving Surfaces; G0872; EPSRC-ENGINEERING & PHYSICAL SCIENCES RESEARCH COUNCIL; EP/J016780/1

Unravelling new mathematics for 3D cell migration; G1438; LEVERHULME TRUST; RPG-2014-149

History

Publication status

  • Published

File Version

  • Published version

Journal

PLoS ONE

ISSN

1932-6203

Publisher

Plos

Issue

3

Volume

10

Article number

e0121090

Department affiliated with

  • Mathematics Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-04-22

First Open Access (FOA) Date

2015-04-22

First Compliant Deposit (FCD) Date

2015-04-18

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