Activity-regulated RNA editing in select neuronal subfields in hippocampus

Balik, Ales, Penn, Andrew C, Nemoda, Zsofia and Greger, Ingo H (2013) Activity-regulated RNA editing in select neuronal subfields in hippocampus. Nucleic Acids Research, 41 (2). pp. 1124-1134. ISSN 0305-1048

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RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, facilitating fine tuning and homeostasis of cellular functions, a similar regulation has not been described for RNA editing. Here, we show that the AMPA receptor R/G editing site is dynamically regulated in the hippocampus in response to activity. These changes are bi-directional, reversible and correlate with levels of the editase Adar2. This regulation is observed in the CA1 hippocampal subfield but not in CA3 and is thus subfield/celltype-specific. Moreover, alternative splicing of the flip/flop cassette downstream of the R/G site is closely linked to the editing state, which is regulated by Ca(2+). Our data show that A-to-I RNA editing has the capacity to tune protein function in response to external stimuli.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Subjects: Q Science
Q Science > QH Natural history > QH0301 Biology
R Medicine > RC Internal medicine > RC0321 Neurosciences. Biological psychiatry. Neuropsychiatry
Depositing User: Andrew Penn
Date Deposited: 07 Oct 2015 09:23
Last Modified: 06 Mar 2017 11:11

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