SciRep2015.pdf (1.31 MB)
A novel long non-coding natural antisense RNA is a negative regulator of Nos1 gene expression
journal contribution
posted on 2023-06-08, 23:58 authored by Sergei Korneev, Mark Maconochie, Souvik Naskar, Elena I Korneeva, Guy Richardson, Michael O'SheaLong non-coding natural antisense transcripts (NATs) are widespread in eukaryotic species. Although recent studies indicate that long NATs are engaged in the regulation of gene expression, the precise functional roles of the vast majority of them are unknown. Here we report that a long NAT (Mm-antiNos1 RNA) complementary to mRNA encoding the neuronal isoform of nitric oxide synthase (Nos1) is expressed in the mouse brain and is transcribed from the non-template strand of the Nos1 locus. Nos1 produces nitric oxide (NO), a major signaling molecule in the CNS implicated in many important functions including neuronal differentiation and memory formation. We show that the newly discovered NAT negatively regulates Nos1 gene expression. Moreover, our quantitative studies of the temporal expression profiles of Mm-antiNos1 RNA in the mouse brain during embryonic development and postnatal life indicate that it may be involved in the regulation of NO-dependent neurogenesis.
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Publication status
- Published
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- Published version
Journal
Scientific ReportsISSN
2045-2322Publisher
Nature Publishing GroupExternal DOI
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1Volume
5Article number
a11815Department affiliated with
- Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2016-01-15First Open Access (FOA) Date
2016-01-15First Compliant Deposit (FCD) Date
2016-01-15Usage metrics
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