GhezziChan.pdf (3.73 MB)
Redox proteomics applied to the thiol secretome
journal contribution
posted on 2023-06-09, 01:06 authored by Pietro Ghezzi, Chan PhilippeSignificance: Secreted proteins are important both as signaling molecules and potential biomarkers. Recent Advances: Protein can undergo different types of oxidation, both in physiological conditions or under oxidative stress. Several redox proteomics techniques have been successfully applied to the identification of glutathionylated proteins, an oxidative post-translational modification consisting in the formation of a mixed disulfide between a protein cysteine and glutathione. Redox proteomics has also been used to study other forms of protein oxidation. Critical Issues: Because of the highest proportion of free cysteines in the cytosol, redox proteomics of protein thiols has focused, so far, on intracellular proteins. However, plasma proteins, such as transthyretin and albumin, have been described as glutathionylated or cysteinylated. The present review discusses the redox state of protein cysteines in relation to their cellular distribution. We describe the various approaches used to detect secreted glutathionylated proteins, the only thiol modification studied so far in secreted proteins, and the specific problems presented in the study of the secretome. Future Directions: This review focusses on glutathionylated proteins secreted under inflammatory conditions and that may act as soluble mediators (cytokines). Future studies on the redox secretome (including other forms of oxidation) might identify new soluble mediators and biomarkers of oxidative stress.
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Publication status
- Published
File Version
- Accepted version
Journal
Antioxidants and Redox SignalingISSN
1523-0864Publisher
Mary Ann LiebertExternal DOI
Issue
7Volume
26Page range
299-312Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2016-05-04First Open Access (FOA) Date
2016-05-06First Compliant Deposit (FCD) Date
2016-05-06Usage metrics
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