Bajwa Paper complete.pdf (1.79 MB)
CMV-specific T-cell responses at older ages: broad responses with a large central memory component may be key to long-term survival
journal contribution
posted on 2023-06-09, 05:04 authored by Martha Bajwa Joseph, Serena Vita, Rosana Vescovini, Martin Larsen, Paolo Sansoni, Nadia Terrazzini, Stefano Caserta, David Thomas, Kevin DaviesKevin Davies, Helen Smith, Florian KernFlorian KernCytomegalovirus (CMV) infection sometimes causes large expansions of CMV-specific T-cells, particularly in older people. This is believed to undermine immunity to other pathogens and to accelerate immunosenescence. While multiple different CMV proteins are recognized, most publications on age-related T-cell expansions have focused on dominant target proteins, UL83 or UL123, and the T-cell activation marker, IFN-?. We were concerned that this narrow approach might have skewed our understanding of CMV-specific immunity at older ages. We have, therefore, widened the scope of analysis to include in vitro-induced T-cell responses to 19 frequently recognized CMV proteins in young and older healthy volunteers and a group of oldest old, long-term survivors (>85 years of age). Polychromatic flow-cytometry was used to analyze T-cell activation markers (CD107, CD154, IL-2, TNF, IFN-?) and memory phenotype (CD27, CD45RA). The older had on average larger T-cell responses than the young, but, interestingly, response size differences were relatively smaller when all activation markers were considered rather than IFN-? or TNF alone. The oldest old recognized more proteins on average than the other groups and had even bigger T-cell responses than the older with a significantly larger central memory CD4 T-cell component.
Funding
The role of CMV-specific immunity in the association between CMV infection and vascular complicatio; G1198; DUNHILL MEDICAL TRUST; R278/0213
History
Publication status
- Published
File Version
- Accepted version
Journal
Journal of Infectious DiseasesISSN
0022-1899Publisher
Oxford University PressExternal DOI
Issue
8Volume
215Page range
1212-1220Department affiliated with
- BSMS Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-02-08First Open Access (FOA) Date
2018-02-13First Compliant Deposit (FCD) Date
2017-02-08Usage metrics
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