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The use of chromatin precipitation coupled to DNA sequencing (ChIP-Seq) for the analysis of Zta binding to the human and EBV genome

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posted on 2023-06-09, 05:38 authored by Anja Godfrey, Sharada Ramasubramanyan, Alison Sinclair
Determining which components of the transcription machinery associate with the viral and cellular genome, and how this changes at specific stages of the viral life cycle is paramount to understanding how the distinct transcriptional programs associated with primary infection, latency, and disease are established and how they are reprogrammed during initiation and execution of the viral lytic replication cycle. Chromatin precipitations linked to next generation DNA sequencing (ChIP-Seq) allow for the interactions of proteins with DNA to be mapped across both viral and cellular genomes. This can be applied to viral and cellular transcription factors, coactivators and corepressors, modified histones, and modulators of chromatin.

History

Publication status

  • Published

Publisher

Springer

Volume

1532

Page range

191-206

Pages

269.0

Book title

Epstein Barr virus: methods and protocols

Place of publication

New York

ISBN

9781493966530

Series

Methods in molecular biology

Department affiliated with

  • Biochemistry Publications

Research groups affiliated with

  • Gene Expression Research Group Publications

Full text available

  • No

Peer reviewed?

  • No

Editors

Janos Minarovits, Hans Helmut Niller

Legacy Posted Date

2017-04-07

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