KrasovecMBE17.pdf (430.13 kB)
Spontaneous mutation rate in the smallest photosynthetic eukaryotes
journal contribution
posted on 2023-06-09, 07:27 authored by Marc Krasovec, Adam Eyre-WalkerAdam Eyre-Walker, Sophie Sanchez-Ferandin, Gwenael PiganeauMutation is the ultimate source of genetic variation, and knowledge of mutation rates is fundamental for our understanding of all evolutionary processes. High throughput sequencing of mutation accumulation lines has provided genome wide spontaneous mutation rates in a dozen model species, but estimates from nonmodel organisms from much of the diversity of life are very limited. Here, we report mutation rates in four haploid marine bacterial-sized photosynthetic eukaryotic algae; Bathycoccus prasinos, Ostreococcus tauri, Ostreococcus mediterraneus, and Micromonas pusilla. The spontaneous mutation rate between species varies from µ?=?4.4 × 10-10 to 9.8 × 10-10 mutations per nucleotide per generation. Within genomes, there is a two-fold increase of the mutation rate in intergenic regions, consistent with an optimization of mismatch and transcription-coupled DNA repair in coding sequences. Additionally, we show that deviation from the equilibrium GC content increases the mutation rate by ~2% to ~12% because of a GC bias in coding sequences. More generally, the difference between the observed and equilibrium GC content of genomes explains some of the inter-specific variation in mutation rates.
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Publication status
- Published
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- Published version
Journal
Molecular Biology and EvolutionISSN
0737-4038Publisher
Oxford University PressExternal DOI
Issue
7Volume
34Page range
1770-1779Department affiliated with
- Evolution, Behaviour and Environment Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-07-27First Open Access (FOA) Date
2017-07-27First Compliant Deposit (FCD) Date
2017-07-27Usage metrics
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