S0907444909023695.pdf (1.25 MB)
Application of general formulas for the correction of a lattice-translocation defect in crystals of a lentiviral integrase in complex with LEDGF.
journal contribution
posted on 2023-06-09, 08:02 authored by Stephen Hare, P Cherepanov, J WangThe symmetry inherent to many biological macromolecular assemblies has been implicated in a range of crystal pathologies, including lattice-translocation defects (LTDs). Crystals suffering from classic LTDs contain two lattices that are shifted with respect to each other but nonetheless remain within the length of coherent interference. LTD introduces an undesirable intensity modulation into diffraction data, resulting in scrambled or partially scrambled electron densities. In this report, LTD theory is extended and a new general method for determining defect fractions is developed based on the heights of the non-origin peaks observed in native Patterson maps. The application of this method to crystals of lentiviral integrase in complex with its cofactor, where the observed translocation vector does not equal a small integral fraction of a unit-cell edge, is reported and its general application to all classic LTD cases is predicted.
History
Publication status
- Published
File Version
- Published version
Journal
Acta Crystallographica Section D: Biological CrystallographyISSN
0907-4449Publisher
International Union of CrystallographyExternal DOI
Issue
Pt 9Volume
65Page range
966-973Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-09-21First Open Access (FOA) Date
2017-09-21First Compliant Deposit (FCD) Date
2017-09-21Usage metrics
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