NatureComms_DNALigaseC.pdf (2.56 MB)
DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria
Version 2 2023-06-12, 08:43
Version 1 2023-06-09, 08:03
journal contribution
posted on 2023-06-12, 08:43 authored by Przemyslaw Plocinski, Nigel Brissett, Julie Bianchi, Anna Brzostek, Malgorzata Korycka-Machala, Andrzej Dziembowski, Jaroslaw Dziadek, Aidan DohertyAidan DohertyProkaryotic Ligase D is a conserved DNA repair apparatus processing DNA double-strand breaks in stationary phase. An orthologous Ligase C (LigC) complex also co-exists in many bacterial species but its function is unknown. Here, we show that the LigC complex interacts with core BER enzymes in vivo and demonstrate that together these factors constitute an excision repair apparatus capable of repairing damaged bases and abasic sites. The polymerase component, which contains a conserved C-terminal structural loop, preferentially binds to and fills-in short gapped DNA intermediates with RNA and LigC ligates the resulting nicks to complete repair. Components of the LigC complex, like LigD, are expressed upon entry into stationary phase and cells lacking either of these pathways exhibit increased sensitivity to oxidising genotoxins. Together, these findings establish that the LigC complex is directly involved in an excision repair pathway(s) that repairs DNA damage with ribonucleotides during stationary phase.
Funding
Molecular basis for repairing DNA double-strand breaks by non homologous end-joining; G0887; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/J018643/1
Cell cycle regulation of the NHEJ DNA double-strand break repair pathway in eukaryotes; G1554; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/M004236/1
Understanding the molecular dynamics of NHEJ-mediated synapsis; BBSRC; BB/F013795/1
History
Publication status
- Published
File Version
- Published version
Journal
Nature CommunicationsISSN
2041-1723Publisher
Nature Publishing GroupExternal DOI
Issue
1Volume
8Page range
1-12Article number
a1251Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-09-22First Open Access (FOA) Date
2017-11-01First Compliant Deposit (FCD) Date
2017-09-22Usage metrics
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