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Computational analysis of miRNA-mediated repression of translation: implications for models of translation initiation inhibition

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journal contribution
posted on 2023-06-09, 08:54 authored by Tracy Nissan, Roy Parker
The mechanism by which miRNAs inhibit translation has been under scrutiny both in vivo and in vitro. Divergent results have led to the suggestion that miRNAs repress translation by a variety of mechanisms including blocking the function of the cap in stimulating translation. However, these analyses largely only examine the final output of the multistep process of translation. This raises the possibility that when different steps in translation are rate limiting, miRNAs might show different effects on protein production. To examine this possibility, we modeled the process of translation initiation and examined how the effects of miRNAs under different conditions might be explained. Our results suggest that different effects of miRNAs on protein production in separate experiments could be due to differences in rate-limiting steps. This analysis does not rule out that miRNAs directly repress the function of the cap structure, but it demonstrates that the observations used to argue for this effect are open to alternative interpretations. Taking all the data together, our analysis is consistent with the model that miRNAs may primarily repress translation initiation at a late step.

History

Publication status

  • Published

File Version

  • Published version

Journal

RNA

ISSN

1469-9001

Publisher

Cold Spring Harbor Laboratory Press

Issue

8

Volume

14

Page range

1480-91

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-11-17

First Open Access (FOA) Date

2017-11-17

First Compliant Deposit (FCD) Date

2017-11-17

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