[Protocol]: Transformation of schizosaccharomyces pombe: electroporation procedure

Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreating S. pombe cells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.