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Murray, Johanne M, Watson, Adam T and Carr, Antony M (2016) [Protocol] Colony polymerase chain reaction with Schizosaccharomyces pombe. Cold Spring Harbor Protocols, 2016 (5). ISSN 1559-6095
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Official URL: http://dx.doi.org/10.1101/pdb.prot090993
Abstract
When screening a large number of individual Schizosaccharomyces pombe strains by polymerase chain reaction (PCR), a rapid “colony PCR” approach may be used. Numerous colony PCR protocols are available, and fundamental to them all is that the colony must be fresh (grown overnight) and that as few cells as possible are used. In this protocol, we present three reliable methods for preparing S. pombe cells for colony PCR.
Item Type: | Article |
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Schools and Departments: | School of Life Sciences > Sussex Centre for Genome Damage and Stability |
Research Centres and Groups: | Genome Damage and Stability Centre |
Subjects: | Q Science > Q Science (General) |
Depositing User: | Sarah Frances |
Date Deposited: | 13 Feb 2018 12:29 |
Last Modified: | 16 Apr 2018 15:25 |
URI: | http://srodev.sussex.ac.uk/id/eprint/72034 |
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📧 Request an updateFunder and Project Information
Project Name | Sussex Project Number | Funder | Funder Ref |
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Smc5/6 and replication fork stability | G0179 | MRC-MEDICAL RESEARCH COUNCIL | G0901011 |
Replication stalling in a palindrome | G0181 | ASSOCIATION FOR INTERNATIONAL CANCER RESEARCH | 10-0273 |