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Transcriptional profiling mycobacterium tuberculosis from patient sputa
chapter
posted on 2023-06-09, 11:46 authored by Leticia Muraro Wildner, Katherine A Gould, Simon WaddellSimon WaddellThe emergence of drug resistance threatens to destroy tuberculosis control programmes worldwide, with resistance to all first-line drugs and most second-line drugs detected. Drug tolerance (or phenotypic drug resistance) is also likely to be clinically relevant over the 6-month long standard treatment for drug-sensitive tuberculosis. Transcriptional profiling the response of Mycobacterium tuberculosis to antimicrobial drugs offers a novel interpretation of drug efficacy and mycobacterial drug-susceptibility that likely varies in dynamic microenvironments, such as the lung. This chapter describes the non-invasive sampling of tuberculous sputa and techniques for mRNA profiling M.tb bacilli during patient therapy to characterise real-world drug actions.
History
Publication status
- Published
File Version
- Accepted version
ISSN
1064-3745Publisher
Humana PressExternal DOI
Volume
1736Page range
117-128Pages
168.0Book title
Antibiotic Resistance ProtocolsISBN
9781493976362Series
Methods in Molecular BIologyDepartment affiliated with
- BSMS Publications
Research groups affiliated with
- Wellcome Trust Brighton and Sussex Centre for Global Health Research Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-01-25First Compliant Deposit (FCD) Date
2018-01-25Usage metrics
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