Bettini,_Natalia_Lorena.pdf (5.96 MB)
NOS-related natural antisense transcripts: sequence analysis and characterization of expression
thesis
posted on 2023-06-07, 16:13 authored by Natalia BettiniEndogenous nitric oxide (NO) produced by the enzyme NO synthase (NOS) has an important role in a variety of physiological processes. However, NO becomes noxious to cells if produced in excess. Therefore, the production of NO is tightly regulated. A particularly exciting and novel aspect of the regulation of NO signalling is the possibility that the expression of NOS genes is controlled by unconventional mechanisms that depend on the presence of natural antisense transcripts (NATs). In this thesis I investigate the important properties of two distinct NOS-related NATs: Lym-antiNOS2 and Mm-antiNOS1. I show that Lym-antiNOS2 RNA is widely expressed in the CNS of the pond snail Lymnaea stagnalis. Furthermore, I demonstrate that the expression of Lym-antiNOS2 is differentially regulated by training leading to long-term memory formation. Moreover, my results indicate that Lym-antiNOS2 RNA is subjected to peripheral trafficking in neurons. As for Mm-antiNOS1, I find that its expression is restricted to embryonic brain tissue and is almost undetectable in the adult brain of Mus musculus.
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- Published version
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209.0Department affiliated with
- Biology and Environmental Science Theses
Qualification level
- doctoral
Qualification name
- dphil
Language
- eng
Institution
University of SussexFull text available
- Yes
Legacy Posted Date
2011-10-18Usage metrics
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