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Intestinal injury and endotoxemia in children undergoing surgery for congenital heart disease

journal contribution
posted on 2023-06-07, 16:23 authored by Nazima Pathan, Margarita Burmester, Tanja Adamovic, Maurice Berk, Keng Wooi Ng, Helen Betts, Duncan Macrae, Simon WaddellSimon Waddell, Mark Paul-Clark, Rosamund Nuamah, Charles Mein, Michael Levin, Giovanni Montana, Jane A Mitchell
Rationale: Children with congenital heart disease are at risk of gut barrier dysfunction and translocation of gut bacterial antigens into the bloodstream. This may contribute to inflammatory activation and organ dysfunction postoperatively. Objectives: To investigate the role of intestinal injury and endotoxemia in the pathogenesis of organ dysfunction after surgery for congenital heart disease. Methods: We analyzed blood levels of intestinal fatty acid binding protein and endotoxin (endotoxin activity assay) alongside global transcriptomic profiling and assays of monocyte endotoxin receptor expression in children undergoing surgery for congenital heart disease. Measurements and Main Results: Levels of intestinal fatty acid binding protein and endotoxin were greater in children with duct-dependent cardiac lesions. Endotoxemia was associated with severity of vital organ dysfunction and intensive care stay. We identified activation of pathogen-sensing, antigen-processing, and immune-suppressing pathways at the genomic level postoperatively and down-regulation of pathogen-sensing receptors on circulating immune cells. Conclusions: Children undergoing surgery for congenital heart disease are at increased risk of intestinal mucosal injury and endotoxemia. Endotoxin activity correlates with a number of outcome variables in this population, and may be used to guide the use of gut-protective strategies.

History

Publication status

  • Published

Journal

American Journal of Respiratory and Critical Care Medicine

ISSN

1073-449X

Publisher

American Thoracic Society

Issue

11

Volume

184

Page range

1261-1269

Department affiliated with

  • Global Health and Infection Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-07-17

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