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Whole genome analysis using microarrays

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posted on 2023-06-07, 16:23 authored by Simon WaddellSimon Waddell, Jason Hinds, Philip D Butcher
The development of microarray technology has allowed the genomes of mycobacteria to be directly compared to identify DNA regions that differ between strains due to deletion, insertion, or sequence divergence. The use of microarrays in comparative genomics has proved to be a valuable tool for comparing both mycobacterial species and strains. We describe here the methodology for comparing two mycobacterial DNA samples by microarray hybridization, from labeling and slide preparation, to DNA microarray analysis options. Further developments in microarray design and methodology promise to ensure that microarrays remain an important resource for comparative genomic studies in the future.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN

1940-6029

Publisher

Springler

Volume

465

Page range

1-12

Book title

Mycobacteria Protocols : Second Edition

Place of publication

Totowa, NJ

ISBN

978-1-58829-889-8

Series

Methods in Molecular Biology

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Editors

Tanya Parish, Amanda Claire Brown

Legacy Posted Date

2012-05-08

First Compliant Deposit (FCD) Date

2017-01-23

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