Novel function of HATs and HDACs in homologous recombination through acetylation of human RAD52 at double-strand break sites

Yasuda, Takeshi, Kagawa, Wataru, Ogi, Tomoo, Kato, Takamitsu A, Suzuki, Takehrio, Dohmae, Naoshi, Takisawa, Kazaya, Nakazawa, Yuka, Genet, Matthew D, Saotome, Mika, Hama, Michio, Konishi, Teruaki, Nakajima, Nakako Izumi, Hazawa, Masaharu, Tomita, Masanori, Koike, Manabu, Noshiro, Katsuko, Tomiyama, Kenichi, Obara, Chizuka, Gotoh, Takaya, Ui, Ayako, Fujimori, Akira, Nakayama, Fumiaki, Hanaoka, Fumio, Sugasawa, Kaoru, Okayasu, Ryuichi, Jeggo, Penny A and Tajima, Katsushi (2018) Novel function of HATs and HDACs in homologous recombination through acetylation of human RAD52 at double-strand break sites. PLoS ONE. pp. 1-38. ISSN 1932-6203

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The p300 and CBP histone acetyltransferases are recruited to DNA double-strand break (DSB) sites where they induce histone acetylation, thereby influencing the chromatin structure and DNA repair process. Whether p300/CBP at DSB sites also acetylate non-histone proteins, and how their acetylation affects DSB repair, remain unknown. Here we show that p300/CBP acetylate RAD52, a human homologous recombination (HR) DNA repair protein, at DSB sites. Using in vitro acetylated RAD52, we identified 13 potential acetylation sites in RAD52 by a mass spectrometry analysis. An immunofluorescence microscopy analysis revealed that RAD52 acetylation at DSBs sites is counteracted by SIRT2- and SIRT3-mediated deacetylation, and that non-acetylated RAD52 initially accumulates at DSB sites, but dissociates prematurely from them. In the absence of RAD52 acetylation, RAD51, which plays a central role in HR, also dissociates prematurely from DSB sites, and hence HR is impaired. Furthermore, inhibition of ataxia telangiectasia mutated (ATM) protein by siRNA or inhibitor treatment demonstrated that the acetylation of RAD52 at DSB sites is dependent on the ATM protein kinase activity, through the formation of RAD52, p300/CBP, SIRT2, and SIRT3 foci at DSB sites. Our findings clarify the importance of RAD52 acetylation in HR and its underlying mechanism.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Depositing User: Penny Jeggo
Date Deposited: 14 Aug 2018 12:55
Last Modified: 14 Aug 2018 12:56

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