Resolving branched DNA intermediates with structure-specific nucleases during replication in eukaryotes

Rass, Ulrich (2013) Resolving branched DNA intermediates with structure-specific nucleases during replication in eukaryotes. Chromosoma, 122 (6). pp. 499-515. ISSN 0009-5915

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Abstract

Genome duplication requires that replication forks track the entire length of every chromosome. When complications occur, homologous recombination-mediated repair supports replication fork movement and recovery. This leads to physical connections between the nascent sister chromatids in the form of Holliday junctions and other branched DNA intermediates. A key role in the removal of these recombination intermediates falls to structure-specific nucleases such as the Holliday junction resolvase RuvC in Escherichia coli. RuvC is also known to cut branched DNA intermediates that originate directly from blocked replication forks, targeting them for origin-independent replication restart. In eukaryotes, multiple structure-specific nucleases, including Mus81-Mms4/MUS81-EME1, Yen1/GEN1, and Slx1-Slx4/SLX1-SLX4 (FANCP) have been implicated in the resolution of branched DNA intermediates. It is becoming increasingly clear that, as a group, they reflect the dual function of RuvC in cleaving recombination intermediates and failing replication forks to assist the DNA replication process.

Item Type: Article
Keywords: Genome stability, chromosome stability, structure-specific nucleases, DNA repair, DNA replication, DNA replication stress, Holliday junction resolvases, Yen1, GEN1, Mus81, Slx1-4
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science
Depositing User: Ulrich Rass
Date Deposited: 24 Sep 2018 13:32
Last Modified: 24 Sep 2018 13:32
URI: http://srodev.sussex.ac.uk/id/eprint/78929

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