Hybrid poly‐l‐lactic acid/poly(ε‐caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell‐derived hepatocyte‐like cells

Mobarra, Naser, Soleimani, Masoud, Ghayour-Mobarhan, Majid, Safarpour, Samaneh, Ferns, Gordon A, Pakzad, Reza and Pasalar, Parvin (2018) Hybrid poly‐l‐lactic acid/poly(ε‐caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell‐derived hepatocyte‐like cells. Journal of Cellular Physiology. ISSN 0021-9541

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Abstract

A suitable alternative strategy for liver transplantation is the use of nanofibrous scaffolds together with stem cells. In this study, a random hybrid of poly-L-lactic acid (PLLA) and polycaprolactone (PCL) was used as a three-dimensional (3D) culture for hepatocyte-like cells (HLCs) differentiation and compared with routine culture (2D). The expression of the endodermal marker, FOXA2, was assessed on day 3 and the hepatic markers; albumin (ALB), alpha-1 antitrypsin (AAT), and cytokeratin-18 (CK-18) were evaluated on day 18 by (qPCR). As well as, albumin, alpha-fetoprotein, and low-density lipoprotein (LDL) uptake were evaluated by immunocytochemistry; moreover Periodic Acid-Schiff and Oil red were done by cell staining. In addition, alpha-fetoprotein (AFP) and urea production were evaluated by chemiluminescence and colorimetric assays. Light and Scanning Electron Microscopy (SEM) microscope showed changes in the cells in 2D and 3D models. The gene expression of hepatic markers werewas significantly higher in the three-dimensional cultures. In addition, immunocytochemistry and cell staining showed that albumin, alpha-fetoprotein, LDL-uptake, Periodic Acid-Schiff, and Oil red were expressed in both cells derived on 2D and 3D. Furthermore, the evaluation of alpha-fetoprotein and urea secretion was significantly different between 2D and 3D strategies. These findings suggest that functionally cells cultured on a PLLA/PCL scaffold may be suitable for cell therapy and regenerative medicine.

Item Type: Article
Keywords: Human induced pluripotent stem cells, Hepatocyte, PLLA/PCL, Scaffold, and Nanofiber
Schools and Departments: Brighton and Sussex Medical School > Brighton and Sussex Medical School
Brighton and Sussex Medical School > Division of Medical Education
Depositing User: Elizabeth Renvoize
Date Deposited: 13 Dec 2018 15:16
Last Modified: 18 Dec 2018 15:30
URI: http://srodev.sussex.ac.uk/id/eprint/80763

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