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Hybrid poly-l-lactic acid/poly(e-caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell-derived hepatocyte-like cells
Version 2 2023-06-12, 08:55
Version 1 2023-06-09, 16:14
journal contribution
posted on 2023-06-12, 08:55 authored by Naser Mobarra, Masoud Soleimani, Majid Ghayour-Mobarhan, Samaneh Safarpour, Gordon FernsGordon Ferns, Reza Pakzad, Parvin PasalarA suitable alternative strategy for liver transplantation is the use of nanofibrous scaffolds together with stem cells. In this study, a random hybrid of poly-L-lactic acid (PLLA) and polycaprolactone (PCL) was used as a three-dimensional (3D) culture for hepatocyte-like cells (HLCs) differentiation and compared with routine culture (2D). The expression of the endodermal marker, FOXA2, was assessed on day 3 and the hepatic markers; albumin (ALB), alpha-1 antitrypsin (AAT), and cytokeratin-18 (CK-18) were evaluated on day 18 by (qPCR). As well as, albumin, alpha-fetoprotein, and low-density lipoprotein (LDL) uptake were evaluated by immunocytochemistry; moreover Periodic Acid-Schiff and Oil red were done by cell staining. In addition, alpha-fetoprotein (AFP) and urea production were evaluated by chemiluminescence and colorimetric assays. Light and Scanning Electron Microscopy (SEM) microscope showed changes in the cells in 2D and 3D models. The gene expression of hepatic markers werewas significantly higher in the three-dimensional cultures. In addition, immunocytochemistry and cell staining showed that albumin, alpha-fetoprotein, LDL-uptake, Periodic Acid-Schiff, and Oil red were expressed in both cells derived on 2D and 3D. Furthermore, the evaluation of alpha-fetoprotein and urea secretion was significantly different between 2D and 3D strategies. These findings suggest that functionally cells cultured on a PLLA/PCL scaffold may be suitable for cell therapy and regenerative medicine.
History
Publication status
- Published
File Version
- Accepted version
Journal
Journal of Cellular PhysiologyISSN
0021-9541Publisher
WileyExternal DOI
Issue
7Volume
234Page range
11247-11255Department affiliated with
- BSMS Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-12-13First Open Access (FOA) Date
2019-12-04First Compliant Deposit (FCD) Date
2018-12-12Usage metrics
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