Kustatscher, Georg, Hégarat, Nadia, Willis, Karen L H, Furlan, Cristina, Bukowski-Wills, Jimi-Carlo, Hochegger, Helfrid and Rappsilber, Juri (2014) Proteomics of a fuzzy organelle: interphase chromatin. The EMBO Journal, 33 (6). pp. 648-664. ISSN 0261-4189
![]() |
PDF
- Published Version
Available under License Creative Commons Attribution. Download (3MB) |
![]() |
PDF
- Accepted Version
Download (3MB) |
Abstract
Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined.
This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate
chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including
1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of CDK regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.
Item Type: | Article |
---|---|
Schools and Departments: | School of Life Sciences > Sussex Centre for Genome Damage and Stability |
Research Centres and Groups: | Genome Damage and Stability Centre |
Subjects: | Q Science > Q Science (General) |
Depositing User: | Sarah Frances |
Date Deposited: | 14 Dec 2018 12:32 |
Last Modified: | 14 Dec 2018 12:32 |
URI: | http://srodev.sussex.ac.uk/id/eprint/80788 |
View download statistics for this item
📧 Request an updateProject Name | Sussex Project Number | Funder | Funder Ref |
---|---|---|---|
Exploiting chemical genetics to investigate the control of microtubule dynamics by mitotic kinases | G0900 | CANCER RESEARCH UK | C28206/A14499 |