A variant in CYP2R1 predicts circulating vitamin D levels after supplementation with high-dose of vitamin D in healthy adolescent girls.

Khayyatzadeh, Sayyed Saeid, Mehramiz, Mehrane, Esmaeily, Habibollah, Mirmousavi, Seyed Jamal, Khajavi, Leila, Salehkhani, Fatemeh Nejati, Hanachi, Parichehr, Bahrami-Taghanaki, Hamidreza, Eslami, Saeed, Vatanparast, Hasan, Ferns, Gordon A, Avan, Amir and Ghayour-Mobarhan, Majid (2018) A variant in CYP2R1 predicts circulating vitamin D levels after supplementation with high-dose of vitamin D in healthy adolescent girls. Journal of Cellular Physiology. ISSN 0021-9541 (Accepted)

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Abstract

Aim: The determinants of serum vitamin D seems to be environmental factors (dietary and supplementary intake and exposure to ultraviolet light) and genetic factors. We aimed to study the relationship between a vitamin D-associated genetic polymorphism and serum 25(OH)D concentrations in healthy adolescent girls in Iran, and its effects on a high dose supplement of vitamin D.
Material and method: A total of 616 healthy adolescent girls with mean age 15 received 50000 IU of vitamin D3 weekly over 9 weeks. Serum vitamin D levels and other metabolic factors were measured at baseline and after the intervention. The genotyping of the CYP2R1 variant (rs10741657) was performed by TaqMan genotyping assays.
Results: Regardless of genetic background, at baseline, 87% of adolescent girls were vitamin D deficient (serum 25(OH)D level<50nmol/l). High-dose supplementation with VitD reduced the proportion of girls who were deficient substantially to about 24%. Genetic analysis revealed that although at baseline there was not a gene-vitamin D association (P trend=0.1), the response to supplementation appeared to be modulated by this variant (P trend<0.001). However, other anthropometric and biochemical measures were not affected by this intervention, over this short period. Serum 25(OH)D was increased in all participants although the carriers of the minor A allele seemed to be better responders so that the percentages of change serum vitamin D in the holder of AA and AG genotypes were 539.4±443.1and 443.7±384.6 respectively, compared to those with common GG genotype (363.3±354.0). Our regression analysis revealed that the probability of an increase in serum 25(OH)D in a participant with AA genotype was 2.5 fold greater than those with a GG genotype (OR=2.5 (1.4-4.4); p value=0.002).
Conclusion: Based on our findings, it appears that the rs10741657 variant of the CYP2R1 gene modulates the response to high-dose of vitamin D supplementation.
Keywords: CYP2R1, rs10741657, vitamin D, supplementation

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Brighton and Sussex Medical School
Brighton and Sussex Medical School > Division of Medical Education
Depositing User: Elizabeth Renvoize
Date Deposited: 09 Jan 2019 17:52
Last Modified: 09 Jan 2019 17:52
URI: http://srodev.sussex.ac.uk/id/eprint/81188

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