s41467-019-10123-1.pdf (7.29 MB)
Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system
Version 2 2023-06-12, 09:04
Version 1 2023-06-09, 17:28
journal contribution
posted on 2023-06-12, 09:04 authored by Haruhisa Okawa, Wan-Qing Yu, Ulf Matti, Karin Scwarz, Benjamin Odermatt, Haining Zhong, Yoshihiko Tsukamoto, Leon LagnadoLeon Lagnado, Fred RIeke, Frank Schmitz, Rachel O L WongRibbon synapses transmit information in sensory systems, but their development is not well understood. To test the hypothesis that ribbon assembly stabilizes nascent synapses, we performed simultaneous time-lapse imaging of fluorescently-tagged ribbons in retinal cone bipolar cells (BCs) and postsynaptic densities (PSD95-FP) of retinal ganglion cells (RGCs). Ribbons and PSD95-FP clusters were more stable when these components colocalized at synapses. However, synapse density on ON-alpha RGCs was unchanged in mice lacking ribbons (ribeye knockout). Wildtype BCs make both ribbon-containing and ribbon-free synapses with these GCs even at maturity. Ribbon assembly and cone BC-RGC synapse maintenance are thus regulated independently. Despite the absence of synaptic ribbons, RGCs continued to respond robustly to light stimuli, although quantitative examination of the responses revealed reduced frequency and contrast sensitivity.
Funding
Synaptic computation in the visual system; G1321; WELLCOME TRUST; 102905/Z/13/Z
History
Publication status
- Published
File Version
- Published version
Journal
Nature CommunicationsISSN
2041-1723Publisher
Nature ResearchExternal DOI
Issue
2167Volume
10Page range
1-13Department affiliated with
- Neuroscience Publications
Research groups affiliated with
- Sussex Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-04-03First Open Access (FOA) Date
2019-05-16First Compliant Deposit (FCD) Date
2019-04-03Usage metrics
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